Purification of a Mouse Monoclonal Antibody Against Erns Protein from Classical Swine Fever Virus

The classical swine fever (CSF) is a high transmission potential disease which produces economical lost in porcine farms from numerous countries. Its causal agent is the classical swine fever virus (CSFV) from which E2 and Erns glycoproteins are the most immunological,1 both used in DIVA (differentiating infected from vaccinated animals) systems to contribute to CSF eradication.2 Serological diagnosis has showed to be a useful tool to diagnostic of CSFV using the Erns glycoprotein as biological marker.3 In Cuba, This protein has been obtained from Escherichia coli Rosetta (DE3) transformed with pET22-Erns and purified by ion metal affinity chromatography (IMAC). To quality controls of the recombinant protein in downstream process is necessary an anti-Erns antibody (mAb). The objective of this work is to obtain a monoclonal anti-Erns antibody and to optimize purification through protein A and G affinity chromatography.