Pseudouridine RNA modification detection and quantification by RT-PCR

Methods（2022）

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摘要

•We describe a method to analyze pseudouridine (Ψ) modifications in mRNA and lncRNA.•The RT-PCR method validates Ψ sites from sequencing or other approaches.•The RT-PCR method quantifies the modification level of Ψ sites.

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关键词

Pseudouridine,CMC,mRNA,lncRNA,RT-PCR

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