Marinobacterium alkalitolerans sp. nov., with nitrate reductase and urease activity isolated from green algal mat collected from a solar saltern

A novel Gram-staining-negative, rod-shaped, 0.6–0.8 µm wide and 2.0–3.0 µm in length, motile bacterium designated strain AK62 T , was isolated from the green algal mat collected from saltpan, Kakinada, Andhra Pradesh, India. Colonies on ZMA were circular, off-white, shiny, moist, translucent, 1–2 mm in diameter, flat, with an entire margin. The major fatty acids include C 16:0 , C 18:1 ω 7 c , and summed feature 3 (C 16:1 ω7c and/or C 16:1 ω6c and/or iso-C 14:0 3-OH). Polar lipids include diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminophospholipid, three unidentified phospholipids, and one unidentified lipid. Polyamine includes Spermidine. The DNA G + C content of the strain AK62 T was 58.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequence revealed that strain AK62 T was closely related to the type strains Marinobacterium sediminicola , Marinobacterium coralli and Marinobacterium stanieri with a pair-wise sequence similarity of 96.9, 96.6 and 96.6%, respectively, forming a distinct branch within the genus Marinobacterium and clustered with M. stanieri , M. sediminicola , M. coralli and M. maritimum cluster. Strain AK62 T shares average nucleotide identity (ANIb, based on BLAST) of 78.44, 76.69, and 76.95% with M. sediminicola CGMCC 1.7287 T , M. stanieri DSM 7027 T , and Marinobacterium halophilum Mano11 T respectively. Based on the observed phenotypic, chemotaxonomic characteristics, and phylogenetic analysis, strain AK62 T is described in this study as a novel species in the genus Marinobacterium , for which the name Marinobacterium alkalitolerans sp. nov. is proposed. The type strain of M. alkalitolerans is AK62 T (= MTCC 12102 T = JCM 31159 T = KCTC 52667 T ).