Nitrogen supply affects cannabinoid and terpenoid profile in medical cannabis (Cannabis sativa L.)

•N Supply affects cannabinoid and terpenoid concentrations in medical cannabis.•Tetrahydrocannabinolic acid and cannabidiolic acid decrease with the increase in N application.•Inflorescence yield is highest under 160–320 mg L−1 N.•Growth retardation and visual chlorosis are induced by N supply lower than 160 mg L−1 N, and N supply up to 320 mg L−1 did not induce a toxicity response.•The optimal N level for yield quantity, combined with relatively high secondary metabolite content, is 160 mg L−1 N.