Functional Changes Of Immune Cells: Signal O Immune Tolerance Of The Ectopic Lesions In Endometriosis?

Research question: What is the potential role of immune cells and their inflammatory cytokines in the pathogenesis, development and establishment of endometriosis?Design: Peritoneal fluid from 59 women (43 with endometriosis and 16 controls) who had undergone laparoscopic surgery was analysed. Changes in the population of innate and adaptive immune cells, cytokines, chemokines and growth factor expression were measured by flow cytometry, Luminex Technology and enzyme-linked immunosorbent assay.Results: No differences were found in the frequencies of the innate and adaptive immune cells between women with and without endometriosis. In the peritoneal fluid of women with endometriosis, IL-1 beta, IL-1RN, IL-2, IL-4, IL-8, IL-10, IL-12 (p70), IL-17 alpha, FGF2, G-CSF, MCP-1, MIP-1 alpha and TNF-alpha were significantly increased compared with controls. A correlation between IL-2, MCP-1, MIP-1 alpha, TNF-alpha and the severity of endometriosis was observed. The concentration of neopterin, a possible biomarker for this disease, was increased in women with endometriosis compared with controls.Conclusions: The functional activity of immune cells seemed to be reduced despite their numbers remaining unchanged. The data indicate that a shift of T-H cytokine profile occurs, which increases the T(H)1 -T(H)2 ratio. This is driven by the increased levels of the cytokines (TNF-alpha and IL-2) in women with severe endometriosis.