Enzymatic production of 3’-sialyllactose in milk

•Enzymatic production of 3’SL directly in milk using Trypanosoma rangeli derived enzymes.•Kinetics of enzymatic conversion in milk is the same as that in aqueous systems.•Determination of activation energy (Ea) for enzymatic trans-sialylation reactions.•Robustness of 3’-SL at elevated temperatures (pasteurization) and freeze drying.