NO-LABEL REAGENT COMBINATION FOR GENE EDITING, AND APPLICATION THEREOF

A no-label reagent combination for gene editing, comprising: (i) a first nucleic acid construct, or a first vector containing the first nucleic acid construct; and (ii) a second nucleic acid construct, or a second vector containing the second nucleic acid construct. By using a nucleic acid construct of a specific structure, the site-directed knock-in and/or replacement of an RNA-guided base is successfully implemented in a plant.