Peripheral Mechanisms Involved In Tityus Bahiensis Venom-Induced Pain

Scorpionism is a public health burden in Brazil. Tityus bahiensis is responsible for most accidents in the Southeastern region of Brazil. Here, the hyperalgesic mechanisms of Tityus bahiensis venom were investigated, focusing on the role of pro-inflammatory cytokines (tumor necrosis factor alpha [TNF-alpha] and interleukin 1 beta [IL-1 beta]) and activation of the transcription factor NFKB. Intraplantar (i.pl.) administration of Tityus bahiensis venom (0.2, 0.6, 1.2 and 2.4 mu g/20 mu L i.pl.) induced mechanical hyperalgesia and thermal hyperalgesia. The 2.4 mu g dose of Tityus bahiensis venom induced overt pain-like behavior and increased myeloperoxidase (MPO) and N-acetylbeta-D-glucosaminidase (NAG) activities, TNF-alpha and IL-1 beta levels in the paw tissue. Systemic pre-treatment with etanercept (soluble TNF-alpha receptor; 10 mg/kg), IL-1ra (IL-1 receptor antagonist; 30 mg/kg) and pyrrolidine dithiocarbamate (PDTC, nuclear factor kappa B [NFKB] inhibitor; 100 mg/kg) inhibited Tityus bahiensis venominduced mechanical and thermal hyperalgesia, MPO and NAG activity and overt pain-like behavior. These data demonstrate the involvement of TNF-alpha and IL-1 beta signaling as well as NFKB activation in Tityus bahiensis venominduced mechanical and thermal hyperalgesia, overt pain-like behavior, and MPO activity and NAG activity, indicating thus, that targeting these mechanisms might contribute to reducing the pain in this scorpionism.