Expression Of Transforming Growth Factor Beta Isoforms In Canine Endometrium With Cystic Endometrial Hyperplasia-Pyometra Complex

Simple Summary Pathomorphological changes and functional disorders of the uterus have long been a significant problem in the reproduction of dogs. The most commonly identified uterine disorders leading to permanent loss of fertility in dogs include cystic endometrial hyperplasia (CEH) and pyometra. These diseases may occur jointly as a CEH-pyometra complex. Despite numerous studies, the etiology of this disease remains unclear. TGF-beta is considered to be one of the key factors in pathophysiological uterine disorders. The results indicate the significant expression of TGF-beta 1 in endometrial tissues in bitches affected by CEH-pyometra complex. Consequently, among all TGF-beta isoforms, TGF-beta 1 is a potential biomarker involved in the regulation of a dog's endometrium with proliferative and degenerative changes. Cystic endometrial hyperplasia (CEH) and pyometra are the most frequently diagnosed uterine diseases affecting bitches of different ages. Transforming growth factor beta (TGF-beta) has been classified in females as a potential regulator of many endometrial changes during the estrous cycle or may be involved in pathological disorders. The aim of this study was to determine the expression of TGF-beta 1, -beta 2 and -beta 3 in the endometrium of bitches suffering from CEH or a CEH-pyometra complex compared to clinically healthy females (control group; CG). A significantly increased level of TGF-beta 1 mRNA expression was observed in the endometrium with CEH-pyometra compared to CEH and CG. Protein production of TGF-beta 1 was identified only in the endometrium of bitches with CEH-pyometra. An increase in TGF-beta 3 mRNA expression was observed in all the studied groups compared to CG. The expression of TGF-beta 2 mRNA was significantly higher in CEH and lower in CEH-pyometra uteri. The results indicate the presence of TGF-beta cytokines in canine endometrial tissues affected by proliferative and degenerative changes. However, among all TGF-beta isoforms, TGF-beta 1 could potentially be a key factor involved in the regulation of the endometrium in bitches with CEH-pyometra complex.