Mitochondrial Fission Related Mirnas Plays A Role In The Etiology Of Pulmonary Artery Diseases

Background: Mitochondria play a crucial role in developing cardiovascular diseases through reactive oxygen species production. Mitochondria are dynamic organelles that undergo fission and fusion and mitochondrial fission is reported to cause pulmonary artery diseases (PH). This study examined whether and how mitochondrial fission-related miRNA are involved in the etiology of PH. Methods: We enrolled 41 patients with PH (mean age: 61.2±13.4, 31 female) and 30 control patients (mean age: 67.4±12.0, 14 female). PH patients were then categorized as group 1 (n=12), group 3(n=7) and group 4 (n=22). We isolated total RNAs from blood samples and measured circulating miR-140 regulating mitochondrial dynamics to fission and miR-30, miR-485 and miR-499 which induce mitochondrial fusion by real-time PCR. Results: The circulating miRNA levels of miR-140-3p was higher and that of miR-485-3p and miR-485-5p were lower in PH compared to Ctr, whereas miR-30 and miR-499 did not change between the two groups. Among PH patients, circulating levels of miR-140 was highest in patients with group1, and that of miR-485was lowest in patients with group3. These results suggest that circulating miRNA which regulate mitochondrial dynamics to fission is related to PH. Moreover, the levels of miR-140 was co-related to SvO2 (p=0.0065) and the change in cardiac index in PH(p=0.0385), indicating that circulating miR-140 may play a crucial role in the etiology and severity of PH. Conclusion: Circulating miRNA which regulate mitochondrial dynamics to fission may Plays a crucial role in developing PH.