Development Of Quantitative Analytical Method For Volatile Thiol Compound With Lc-Esi-Ms As Nonvolatile Derivative By Integrating A Thiol-Specific Derivatization

Generally, volatile thiols are hard to be measured with electrospray-ionization-type LC-MS due to the volatility. Therefore, we here evaluated the pretreatment of their S-bimanyl derivatization by monobromobimane to enable the detection as nonvolatile derivative. Consequently, we successfully developed the convenient and efficient method through the quantitative analysis of 2-furanmethanethiol (volatile thiol odorant of coffee aroma) in coffee bean.