Diversion of Food Waste into the Sulfate-Laden Sewer: Interaction and Electron Flow of Sulfidogenesis and Methanogenesis.

Diverting food waste (FW) into the sulfate-laden sewer may pose a significant influence on the production of methane and sulfide in sewers. Identifying microbial electron utilization is essential to understanding the interaction of sulfidogenesis and methanogenesis in depth. Here, we reported sulfide and methane production from the sewer bioreactors receiving sulfate-laden wastewater (160 mg S/L), with and without FW addition. Long-term monitoring showed that the addition of FW (1 g/L) could boost both sulfide (by 39%) and methane (by 44%) production. As for the electrons used for sulfidogenesis and methanogenesis, about 98% flowed to sulfidogenesis. Cryosection-fluorescence in situ hybridization showed that high sulfate content suppressed the accumulation of methanogens in biofilm outer layer, whereas methanogens in the inner layer were enriched with FW addition. Moreover, the FW addition fostered the diversity of the fermentative bacteria and changed the type of methanogens in biofilms, and up-regulated the key enzymes expressions for sulfidogenesis and methanogenesis. A model-based investigation suggests that increased FW-to-sewage ratios would exert a significant impact on methane production than on sulfide production. The microbial electron flows were highly dependent on sulfate concentration and FW-to-sewage ratios. The findings of this study suggest that sulfate and substrate levels play a key role in microbial electron utilization for sulfide and methane production, and diverting FW into the sulfate-laden sewer may exert negative impacts on sewer management and the environment.