A streamlined CRISPR workflow to introduce mutations and generate isogenic iPSCs for modeling amyotrophic lateral sclerosis

Eric Deneault,Mathilde Chaineau,Michael Nicouleau,Maria José Castellanos Montiel,Anna Kristyna Franco Flores,Ghazal Haghi,Carol X-Q Chen,Narges Abdian,Irina Shlaifer,Lenore K Beitel,Thomas M Durcan

Methods（2022）

引用 13|浏览11

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摘要

•Improved protocol for point mutation substitution in human iPSCs.•Correction of I114T in SOD1.•Insertion of G94A in SOD1 and H517Q in FUS.•Differentiation of iPSCs into motor neurons.

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关键词

CRISPR,Isogenic iPSC,ALS,SOD1-I114T,SOD1-G94A,FUS-H517Q

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