Detailed Methodology to Establish a Synovium/Cartilage-on-a-Chip Model to Investigate the Process of Monocyte Extravasation.
Methods in molecular biology (Clifton, N.J.)(2022)
摘要
Microfluidics allows for recapitulating organotypic environments in miniaturized cell culture platforms. This ability paves the way to the investigation of complex biological processes in a relevant milieu. Here we describe the protocols to generate an organotypic model including a vascularized compartment mimicking the synovial membrane and designed for the study of monocyte extravasation during osteoarthritis.
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关键词
Cartilage,Extravasation,Microfluidics,Monocyte,Organ-on-a-chip,Synovium
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