Effect Of Feed Containing Hermetia Illucens Larvae Immunized By Lactobacillus Plantarum Injection On The Growth And Immunity Of Rainbow Trout (Oncorhynchus Mykiss)

Simple Summary In this study, we evaluated the effect on the growth and immunity of rainbow trout of a feed formulated using Hermetia illucens with increased antimicrobial peptides expression by Lactobacillus plantarum infection (ImHIL). As a result, growth and immunological indicators improved, and therefore, ImHIL is expected to become a good feed source for rainbow trout aquaculture. We investigated the effects of a feed containing Hermetia illucens larvae injected with bacteria on the growth and immunity of Oncorhynchus mykiss. The feed was prepared by replacing fishmeal in feed with 25 and 50% nonimmunized (HIL25, HIL50) or immunized HIL (ImHIL25, ImHIL50), and its protein:fat:carbohydrate ratio was 45:15:18. ImHIL extracts showed inhibitory activity against fish pathogenic bacteria. Both red blood cell count and insulin-like growth factor-1 as the growth indicator were the highest among the groups at week 6 after feeding in the ImHIL50 group. As immune indicators, blood aspartate aminotransferase levels were lower in the ImHIL25 and ImHIL50 groups than in that of other groups at week 6 after feeding, and lysozyme content was significantly higher in ImHIL25 and ImHIL50. The above results demonstrate that ImHIL has a beneficial effect on the improvement of growth and immunity. Accordingly, we suggest that ImHIL has the potential to be a good feed source in aquaculture.