Characterization of macrophage infiltration and polarization by double fluorescence immunostaining in mouse colonic mucosa.

We recently characterized the association between DNA damage and immunoresponse in colonic mucosa of mice infected with a Typhimurium strain expressing a genotoxin, known as typhoid toxin. In this protocol, we describe how to assess the extent and features of infiltrating macrophages by double immunofluorescence. Total macrophage population was determined using an F4/80 antibody, whereas the specific M2-like population was assessed using a CD206 antibody. For complete details on the use and execution of this protocol, please refer to Martin et al. (2021).