Combining Ancestral Reconstruction with Folding-Landscape Simulations to Engineer Heterologous Protein Expression

Gloria Gamiz-Arco,Valeria A Risso,Eric A Gaucher,Jose A Gavira,Athi N Naganathan,Beatriz Ibarra-Molero,Jose M Sanchez-Ruiz

Journal of Molecular Biology（2021）

引用 4|浏览11

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摘要

•Obligate symbionts exhibit high evolutionary rates and extensive sequence divergence.•Protein from obligate symbiont displays impaired folding outside its original host.•Protein design based on ancestral reconstruction and folding landscape simulations.•Sequence engineering approach to rescue inefficient heterologous expression.•Potential application in metagenomics efforts targeting recent adaptations.

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关键词

ancestral sequence reconstruction,computational modelling of protein folding landscapes,heterologous protein expression,proteins from uncultured organisms,obligate symbionts

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