Robust Functional Imaging Of Taste Sensation With A Bessel Beam

Functional imaging of intact taste cells in response to various tastant solutions poses a technical challenge since the refractive index of the immersion medium dynamically changes during tastant delivery. Critically, the focal shift introduced by high-index tastant solutions has been the fundamental limit in experimental design. Here we seek to address this issue by introducing an axially elongated Bessel beam in two-photon microscopy. Compared to the conventional Gaussian beam, the Bessel beam provides superior robustness to the index-induced focal shift, allowing us to acquire near artifact-free imaging of taste cells in response to a physiological taste stimulus. (c) 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement