Hydroxytyrosol effectively improves the quality of pig sperm at 17 degrees C

Artificial insemination (AI) is a proven breeding technology which has been widely used in pig reproduction. Low temperature can cause very serious damage to pig sperm below 15 degrees C and the situation is even more serious at lower temperature. Besides, the preservation of pig sperm is mainly carried out at 17 degrees C because of its outstanding performance in pig reproduction. However, the accumulation of a large amount of reactive oxygen species (ROS) during the preservation process is the main reason for the deterioration of sperm quality. In our research, by adding different concentrations of hydroxytyrosol to the diluent during the storage of pig sperm at 17 degrees C, we compared them with the traditional diluent to study the sperm motility, the cumulative amount of ROS, the extent of sperm membrane damage, the sperm acrosome integrity, the sperm DNA damage and the activity of antioxidant enzymes (CAT, T-AOC, SOD, GSH-PX, MDA) to evaluate the effect of hydroxytyrosol on the sperm quality during storage. We used proteomics sequencing technology to monitor difference in sperm protein between the control samples and the addition of 120 mu mol/L hydroxytyrosol samples (optimum concentration) after three days storage. Ultimately, we selected the control samples and the addition of 120 mu mol/L hydroxytyrosol samples to test the effect of AI. The results of our research showed that during storage of pig sperm at 17 degrees C, the sperm quality and antioxidant capacity of the hydroxytyrosol-treated samples significantly improved (HT 120 mu mol/L) (P < 0.05). Proteomics sequencing analysis proved that the addition of 120 !Imola. hydroxytyrosol treatment samples had potential value in improving sperm quality. The significant increase in sow pregnancy rate and piglet birth weight proved that hydroxytyrosol had important practical value in pig reproduction. Based on our results, we demonstrated that the addition of hydroxytyrosol to the diluent could improve the quality of pig sperm and the efficiency of AI. (C) 2021 Published by Elsevier Inc.