High-Dimensional Tissue Profiling by Multiplexed Ion Beam Imaging.
Methods in molecular biology (Clifton, N.J.)(2022)
摘要
Multiplexed Ion Beam Imaging by Time of Flight (MIBI-TOF) enables high-dimensional imaging in situ of clinical specimens at single-cell resolution. In MIBI-TOF, tissue sections are stained with dozens of metal-labeled antibodies, whose abundance and location are read by secondary ionization mass spectrometry. The result is a multi-dimensional image, depicting sub-cellular expression and localization for dozens of distinct proteins in situ. Here, we describe the staining and imaging procedures of a MIBI-TOF experiment.
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关键词
High-dimensional imaging,Histology,Imaging,MIBI-TOF,Mass spectrometry,Multiplexed imaging,Multiplexed ion beam imaging,Pathology,Proteomics,Single cell
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