Transposon Tagging In Setaria Viridis

Transposon tagging populations are important resources for characterizing gene function in model plant systems. In Setaria viridis, a transposon tagging population utilizing the miniature Ping (mPing) element was generated by introducing a construct containing mPing and the transposase Ping into accession A10.1. Transformants were screened for the presence of the transgene and mPing insertions were identified using nested inverse-PCR coupled with Illumina sequencing. Here we describe step-by-step protocols for generating the Setaria viridis mPing population, introduce methods for genotyping mPing insertion alleles, and discuss future implementations of the system.