Simple Sequence Repeat

DNA markers are powerful tools for molecular breeding or checking the genetic homogeneity of F1 hybrid seeds. For the simple and fast approach to detect DNA polymorphisms, simple sequence repeats (SSRs), which are tandem repeats of short sequence motifs (mainly 1-6 bases), are powerful markers because of their codominant mode of inheritance, higher reliability, and abundance in genomes. In Allium, GT/CA repeats are abundant than GA/CT repeats, and AAG/CTT motif is the most frequent in the trinucleotide SSRs. NGS technologies including public sequence data will be a mainstream for identifying new SSR markers.