Study On The Tissue Tropism Of Canine Parvovirus Based On The Distribution Of The Transferrin Receptors

Canine parvovirus (CPV-2) emerged as a pathogen of new acute infectious disease in dogs. It affects primarily young animals where it can cause haemorrhagic enteritis and myocarditis. Little is known about the mechanism of pathogeneris virulence of CPV-2. Receptor binding is a key step in the life cycles of all animal viruses, and in many cases the virus-receptor interactions determine both the host susceptibility and tissue tropism. It has been reported that the specific binding to transferrin receptor on cell membranes play an important role in tissue tropism and host ranges of CPV-2. In this study, we aimed to correlate the distribution of TfR with CPV-2 infectious capability on primary tissue cultures. Our results demonstrated that, CPV-2 can reproduce in primary culture cells of liver, kidney, cardiac muscle, spleen and intestinal epithelia but for lung, and the distribution of tranferrin receptor in primary cells was relative to the culture tropism of CPV-2. These results confirmed that the TfR could be critical for the infection of CPV-2 in primary tissue cells from fetal tissue.