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Phylogenetic Relationship Among Selected Wild And Cultivated Fabaceae Species

BIOSCIENCE RESEARCH(2020)

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Abstract
The phylogenetic and comparative analysis of the Fabaceae family is an important and powerful tool for legume plant breeders since it identifies close relatives as focal points for legume crop improvement and also provides information on wild relatives of legumes crop species. The present study was designed to examine the phylogenetic relationship amongst the selected species of family Fabaceae. For the estimation of phylogenetic relationship among 160 genotypes of family Fabaceae belonging to 8 different species. Both morphological and biochemical characterizations were explored. Data were analyzed using computer software SPSS and PC-ord5. In SDS-PAGE analyses, nine loci were recorded; out of the nine loci, B-9 was missing in V. unguiculata and V. radiata R. rothii and A. platycarpa, B-1and B-9 were absent V. radiata var. sublobata while B-7 & 9 were absent in R. capitata. The absence or missing band could be helpful in the identification of these species. The interspecies locus contribution was 88.888%. Locus/band 6 (B-6) was present in all collected genotypes of the current study. The current data reveal ample intra and inter-species genetic diversity within these eight species, each species maintaining species-specific individuality in the area irrespective of environmental fluctuation. The presence of Locus B-6 in all the collected genotypes was considered as a family-specific locus suggest their close genetic similarity and common heritage, and was considered as a family-specific locus for the selected species.
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Key words
Fabaceae, Morphology, SDS-PAGE, Phylogeny, Cluster analysis
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