Micellar Electrokinetic Capillary Chromatographic Separation Of Pyrimidine And Purine Derivatives

The retention behaviours of 12 purine and pyrimidine derivatives - - thymine, uracil, cytosine, adenine, guanine, xanthine, hypoxanthine, purine, 4,6-diaminopyrimidine, theophylline, trimethoprimum and 6-(benzylamino)purine, have been studied by micellar electrokinetic capillary chromatography(MECC). 10 out of 12 analytes were separated by using 40 mmol/L sodium dodecyl sulfate - 10 mmol/L borax - 17% methanol-3.0 mmol/L beta-cyclodextrin (pH 9.30) as electrolyte. The relative standard deviations (RSD) of t(R) and UV absorbance were less than 1.8% and 6.5% (n=6), respectively.