The Value Of Aspl-Tfe3 Dual-Color Single-Fusion Fish Assay In Diagnosing Alveolar Soft Part Sarcoma

Objectives: To improve the accuracy of the diagnosis of alveolar soft part sarcoma, ASPL-TFE3 dual-color, single-fusion fluorescence in situ hybridization (FISH) probe was developed, especially for alveolar soft part sarcoma with atypical morphological features, unfamiliar positions or overlap of its pathological features with other tumors. Methods: ASPL-TFE3 dual-color, single-fusion FISH probe was invented for the diagnosis of alveolar soft part sarcoma. The probe was performed on formalin-fixed, paraffin-embedded tissues from ten alveolar soft part sarcoma, five granular cell tumors, five rhabdomyosarcoma and five paraganglioma in our hospital. All the cases were restudied, including pathological morphology and immunohistochemistry, and TFE3 immunohistochemistry and FISH assay were taken for all the cases. Results: TFE3 Immunohistochemistry was positive in ten alveolar soft part sarcoma cases and one of paraganglioma. ASPL-TFE3 single-fusion FISH assay was positive in all alveolar soft part sarcoma, and negative in granular cell tumor, rhabdomyosarcoma and paraganglioma cases. Conclusions: This FISH assay can precisely detect the ASPL-TFE3 fusion gene, and it can be utilized as a useful adjunct way for the diagnosis of alveolar soft part sarcoma.