Comparative Analysis Of Transcriptomes From Synthetic Cpg Odn/Cpg Free Dna Stimulated Bovine Pbmcs

It has been reported that both cytosolic DNA and the single-stranded synthetic phosphorothioate oligodeoxynucleotides containing specific CpG motifs (CpG ODN) could activate the host innate immune system and induce up-regulation of the expression of Type I interferons (IFNs) and pro-inflammatory cytokines, as well as the subsequent induction ISGs. Albeit the similarity in innate immune activation, cytosolic DNA is reported to be recognized and signal through the cGAS-STING-TBK1 axis, while CpG ODN engages with endosomal TLR9. Until recently, few data were available on the transcriptomes of cytosolic DNA or synthetic CpG ODN stimulated PBMCs. In the current study, bovine PBMCs were stimulated with CpG Free DNA or CpG ODN, respectively. RNA-seq technology was employed to study the blood gene expression profiles from the PBMCs treated with each stimulus. DEGs were identified, and the up-regulated DEGs were run through GO and KEGG analysis. The similarities as well as differences between CpG Free DNA or CpG ODN stimulated transcriptomes were compared. Our data provide new information on nucleic acids-drived immune activation and shed light on the use of different forms of nucleic acids as potential adjuvants for bovine vaccines. (C) 2019 Friends Science Publishers