Hemodynamic Study About Laminar Shear Stress Extenuate Cerebral Ischaemia-Reperfusion Injury

Objective: In this study, we explored whether laminar shear stress (LS) had an effect on the apoptosis of rat brain microvascular endothelial cells (rBMECs) during the course of cerebral ischaemia-reperfusion injury (CIRI). Methods: First, blood flow changes in CIRI were studied by Laser Doppler flowmetry in vivo. Then, the oxygen/glucose deprivation (OGD) model was adopted to cause ischaemia in rBMECs for 6 hours in vitro. Flow intervention without nutrition and an LS force magnitude of 0.8 +/- 0.05 dynes/cm(2) were delivered for the last 2 hours to simulate the single factor effect of LS on reperfusion. Results: We found that cell apoptosis was significantly relieved under haemodynamic intervention with Annexin V-FITC/PI and Hoechst 33258 analysis. In terms of molecular mechanisms, it is possible that LS could activate the cytomembrane surface receptor Tie-2 and cause downstream inhibition of the caspase-3 apoptosis pathway. Conclusion: LS should be a protective factor for CIRI.