Evaluation of Methodology for Separation and Identification of Tetraniliprole in Maize (zea Mays) Using RP-HPLC Intended for Residual Analysis

The aim of this study was to develop and optimize an RP-HPLC technique for the residue determination of tetraniliprole and its metabolite (BCS-CQ 63359). Standardization was carried out by using simplified quick, easy, cheap, effective, rugged and safe (QuEChERS) method in maize (Zea mays L.) leaves (fodder) and seeds. The optimized method was validated to fulfill the requirements of SANTE/11813/2017 guidelines including selectivity, linearity, precision and accuracy of detection system. A linearity relationship (R-2>0.99) between concentration of tetraniliprole/metabolite and peak area over concentration range was observed. Method used reversed phase C-18 column (5 mu m, 250 mm x 4.6 i.d.) along with photodiode array detector with isocratic mobile phase consisting of ACN: H2O. The flow rate was 0.8 ml/min. and detection gave response at 215 nm. The RSD of peak area ranged from 0.35 to 5.63 % within analytical day and from 1.27 to 4.27 % across analytical days. Limit of detection (LOD) and limit of quantification (LOQ) for both analytes were found to be 0.01 and 0.05 tg/ml respectively. Overall, the results demonstrate that the proposed method can be effectively implemented for screening and quantization of tetraniliprole and its metabolite as active ingredient taking maize as a matrix.