Pattern-Type Separation Of Triacylglycerols By Silver Thiolatexnon-Aqueous Reversed Phase Comprehensive Liquid Chromatography
SEPARATIONS(2021)
摘要
Triacylglycerols (TAGs), as the main components of edible oils and animal fats, are responsible for the nutritional value, organoleptic features and technological properties of foods; each lipid matrix shows a unique TAG profile which can serve as fingerprint to ensure the quality and authenticity of food products. The high complexity of many foodstuffs often makes untargeted elucidation of TAG components a challenging task; thus, more efficient separation techniques may be mandatory. In this research, the TAG profile of a borage (Borago officinalis) seed oil was obtained by two-dimensional comprehensive liquid chromatography (LCxLC), by the coupling of silver thiolate and octadecylsilica monodisperse materials. A total 94 TAG compounds were identified by ion trap-time of flight detection, using atmospheric pressure ionization, with the degree of unsaturation varying from 0 to 9, and partition values ranging from 36 to 56. The group-type separation afforded by this analytical approach may be useful to quickly fingerprint TAG components of oil samples.
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关键词
borage, lipids, triacylglycerols, PUFA, linoleic acid, silver-ion chromatography, NARP-UHPLC, comprehensive liquid chromatography, APCI-MS
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