H-1, C-13 And N-15 Resonance Assignment Of Human Guanylate Kinase

Human guanylate kinase (hGMPK) is a critical enzyme that, in addition to phosphorylating its physiological substrate (d)GMP, catalyzes the second phosphorylation step in the conversion of anti-viral and anti-cancer nucleoside analogs to their corresponding active nucleoside analog triphosphates. Until now, a high-resolution structure of hGMPK is unavailable and thus, we studied free hGMPK by NMR and assigned the chemical shift resonances of backbone and side chain H-1, C-13, and N-15 nuclei as a first step towards the enzyme's structural and mechanistic analysis with atomic resolution.