Study On The Influence Of Urine And Skin Microbiota In The Pathogenesis Of Male Genital Lichen Sclerosus

You have accessJournal of UrologyTrauma/Reconstruction/Diversion: Urethral Reconstruction (including Stricture, Diverticulum) I (MP03)1 Sep 2021MP03-08 STUDY ON THE INFLUENCE OF URINE AND SKIN MICROBIOTA IN THE PATHOGENESIS OF MALE GENITAL LICHEN SCLEROSUS Zeyu Wang, Kaile Zhang, Tao Liang, Zhenghao Dai, Jiong Zhang, Yuemin Xu, Qiang Fu, and Lujie Song Zeyu WangZeyu Wang More articles by this author , Kaile ZhangKaile Zhang More articles by this author , Tao LiangTao Liang More articles by this author , Zhenghao DaiZhenghao Dai More articles by this author , Jiong ZhangJiong Zhang More articles by this author , Yuemin XuYuemin Xu More articles by this author , Qiang FuQiang Fu More articles by this author , and Lujie SongLujie Song More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000001964.08AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Lichen sclerosus (LS) is a chronic inflammatory cicatricial skin lesion which is prone to the external genitalia. In severe cases, it can lead to urethral stricture or even malignant transformation. The etiology is still unclear. Considering its characteristics of chronic inflammation, this study comparatively analyzed the diversity and bacterial colony composition of urine and balanopreputial swab microbiota between male LS urethral stricture and non-LS urethral stricture patients. METHODS: We prospectively included 10 male patients with LS urethral stricture and 10 male patients with non-LS urethral stricture into this case-control study. All patients were adult males and had undergone circumcision or with no history of excessive prepuce. Patients with clear urinary tract infection were excluded. We collected first voiding urine samples and balanopreputial swabs from all included patients except one patient only with balanopreputial swab. After DNA extraction, the 16S V3-V4 hypervariable region was amplified through PCR and sequenced. Operational taxonomic units (OTUs) were defined using a > 97% sequence similarity threshold. We compared the differences in Alpha diversity (numbers and distribution of OTUs), Beta diversity and specific species in the urine and balanopreputial swabs between two groups. RESULTS: Whether in balanopreputial swab or urine, there was no significant difference in Alpha diversity between the two groups (p >0.05). Beta diversity analysis showed that in PCA, PCoA, and hierarchical cluster analysis, it was difficult to distinguish the two groups significantly. From genus level, in the swabs and urine samples of two groups, the flora with higher abundance were not only Corynebacterium, Streptococcus, Lactobacillus and other normal bacteria, but also Klebsiella and Escherichia - Shigella, two classic nosocomial infection bacteria. In the balanopreputial swabs, the abundance of Bacteroidales in the LS group was significantly lower than that in the non-LS group (p=0.013), while the abundance of Collinsella and Lactobacillales in the LS group was significantly higher (p=0.014; 0.048). CONCLUSIONS: There is no significant difference in microbiota Alpha diversity between patients with LS urethral stricture and non-LS urethral stricture whether it is in the local male genital or the urine. Even if patients with urinary tract infection were excluded, the detection rate and abundance of nosocomial pathogens in two groups were still relatively high, which suggested that regardless of the cause, anti-infection management should not be ignored in anterior urethral stricture patients. The occurrence and development of LS may be related to the imbalance of microbiota. The balanopreputial swabs can better reflect this correlation. However, further research is needed to confirm its internal connection and hidden mechanism. Source of Funding: N/A © 2021 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 206Issue Supplement 3September 2021Page: e24-e24 Advertisement Copyright & Permissions© 2021 by American Urological Association Education and Research, Inc.MetricsAuthor Information Zeyu Wang More articles by this author Kaile Zhang More articles by this author Tao Liang More articles by this author Zhenghao Dai More articles by this author Jiong Zhang More articles by this author Yuemin Xu More articles by this author Qiang Fu More articles by this author Lujie Song More articles by this author Expand All Advertisement Loading ...