Comparative And Co-Relative Analysis Of Different Tests For Rapid Diagnosis Of Tuberculosis (Tb) From Pleural Effusion

The present study was aimed to determined and investigate the rapid diagnosis of tuberculosis (TB) by comparing liquid culture, Xpert MTB/RIF assay and adenosine deaminase assay (ADA) methods. A total of 55 pleural effusion samples were collected from patients of 10-75 years of age of both sexes. Mycobacterium tuberculosis and rifampicin resistance Mycobacterial strain was detected by LED fluorescence microscope, Lowenstein-Jensen (L-J) culture, liquid culture, ADA and Gene Xpert MTB/RIF methods. Rapid and accurate diagnosis was estimated by comparative efficiency of Gene Xpert MTB/RIF, ADA and Liquid culture. A total of 20 (36.75%) cases were found positive in Xpert MTB/RIF assay among which 5 (50%) were rifampicin resistant. As compared with the conventional method and liquid culture method, Xpert MTB/RIF showed high sensitivity (100%) and 99% and 98% specificity, respectively. Adenosine diamine level was also counted to determine the TB positivity. Out of 55 patients, 16 (29.09%) were positive for ADA with higher sensitivity specificity. We concluded that, Xpert MTB/RIF assay was evident to be the rapid and accurate method to detect pleural TB, so that patient can get early treatment.