Antifungal Potential Of Endophytic Cladosporium Sphaerospermum L. Isolated From Citrus Medica L.

The purpose of this study was to screen and evaluate the biological potential of secondary metabolites obtained from endophytic Cladosporium sphaerospermum isolated from stem of medicinal plant Citrus medica. Antifungal assay was evaluated from crude extract of endophytic fungal strain. The crude extract isolated from endophytic fungi showing potential activity against bacteria and fungi. The fungal crude extract showed potential activity at higher concentration 500 mu g/mL inhibited the growth of R. stolonifur (10 mm), A. niger (9.9mm) P. chrysogenum (8.3mm) and T. horzianum (7.8mm) respectively. While lower zone of inhibition was observed at 20 mu g/mL concentration against the pathogenic fungal strains i.e T. horzianum (2.3mm), P. chrysogenum (5.5mm) and A. niger (6.3mm) and no inhibition was recorded in the R. stolonifur (0). LCMS analysis showed that the crude extract have the presence of bioactive secondary metabolites. From the present study it is concluded that the crude extract obtained from endophytic fungi Cladosporium sphaerospermum contain bioactive compounds that have the potential to inhibit the growth of pathogenic bacteria and fungi. So it can be used as potent antifungal upon further purification and exploitation.