ATTENUATION OF VISCERAL HYPERSENSITIVITY DUE TO TNBS-INDUCED ILEITIS BY ELECTROACUPUNCTURE, Nigella sativa, Eugenia caryophyllata OIL AND SURGICAL RESECTION OF ILEUM IN DOGS

The study aimed to document the therapeutic effect of application of Electroacupuncture (EA), oral administration of Nigella sativa (NS), Eugenia caryophyllata (EU) oils and surgical resection of ileum on TNBS induced ileitis with visceral hypersensitivity (VH) in dogs.I ml solution containing 30 mg of TNBS in 30 % ethanol was injected into ileal wall of dogs of TNBS group, TNBS + EA group, TNBS + NS group and TNBS + EU group. In saline group, an equal quantity of saline was injected instead of TNBS solution. Dogs in the EA group were treated with EA at day 6, 9, 12, 15, 18 and 21 after TNBS treatment while dogs in TNBS + NS and TNBS + EU group were orally administered with NS oil and EU oil @2.5 ml/kg on day 6, 9, 12, 15, 18 and 21. VH in dogs of all groups was reflected by Pain Response Score (PRS). PRS was assessed by monitoring Behavioral Pain Responses to Colorectal Distension at day 6, 9, 12, 15, 18 and 21 in all groups. Blood samples were taken from each group of dogs on day 6, 9, 15, 18 and 21 after colorectal distension test to measure the Plasma IL-6 Concentrations. Dogs were passed through laparotomy procedure to get ileal tissue samples from each group. Macroscopic and microscopic scoring of each sample was done. Three dogs from saline group (n=3) and three dogs from TNBS group (n=3) were further tested to see the effect of surgical resection of ileum on VH. After induction of ileitis with TNBS/Ethanol dogs exhibited the signs of Vomiting, diarrhea, and decreased body weight. Macroscopic and Microscopic scoring of ileal tissue was increased, plasma concentration of IL-6 was increased and Pain response score in response to colorectal distension was also increased. EA application, oral administration of NS and EU oil relieved the VH by reducing the Pain behavior response, Microscopic and Macroscopic scoring and plasma concentration of IL-6. Surgical resection of effected part of ileum also relieved the VH.