Tnbs-Induced Ileitis With Visceral Hypersensitivity In Dogs

Sixty clinically healthy dogs were divided into two groups i.e., saline (n=30) and TNBS group (n=30). One mL of ethanol mixed TNBS solution (30 mg TNBS in 30% ethanol) was injected in the ileal wall in each dog of the TNBS group after performing laparotomy. In the saline group, one mL of normal saline was injected in the same way. The severity of the inflammatory response was assessed by macroscopic, microscopic scoring, and plasma level of interleukin (IL-6). Visceral hypersensitivity (VH) in these dogs was reflected by pain response score (PRS) due to colorectal distension (CRD) by inserting colorectal distension device on day 2, 7, 13, 19, and 25. TNBS treated dogs showed transmural pathologic changes as compared to the saline-treated group. A significant increase (P < 0.05) in microscopic, macroscopic scores and plasma IL-6 level was observed in the TNBS treated group as compared to the saline group. Dogs showed apparent ileitis from day 2 to 19 and VH was observed from day 7 to 25. The experiment successfully established a reproducible ileitis model in dogs with VH that will help to further study the pathogenesis of IBD and the effect of different therapeutics to treat IBD.