Anti-inflammatory and Cytotoxicity activities of Green Synthesized Silver Nanoparticles from Stem Bark of Terminalia brownii

The present study focused on synthesizing, characterization, and evaluating an in vitro anti-inflammatory and cytotoxic potential of green synthesized silver nanoparticles (TB-Ag NPs) from aqueous stem bark extract of Terminalia brownii (TB-AQ). The TB-Ag NPs were characterized by ultraviolet (UV)-visible, Fourier-transform infrared spectroscopy (FTIR), dynamic light X-ray diffraction, and energy-dispersive X-ray spectroscopy, as well as scanning and transmission electron microscopy. The in vitro anti-inflammatory and cytotoxic potential of TB-Ag NPs and TB-AQ were evaluated against RAW 264.7 macrophage and MDA-MB-231 triple-negative breast cancer cells, respectively, by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Further, the inhibitory effect on LPS-induced production of inflammatory mediators PGE 2 and NO in RAW 264.7 cell lines was evaluated. The results showed that TB-Ag NPs were crystalline with face-centered spherical polydispersed shaped nanoparticles with an average size between 20 and 67 ± 0.5 nm. Also, TB-Ag NPs had no cytotoxic effect on RAW cells (normal healthy cells) in the range of 6.25–50 µg/mL. Besides, TB-Ag NPs at 50 µg/mL concentration exhibited 67.02% of cytotoxic effect against MDA-MB-231 cells with observed IC 50 values of 29.08 µg/mL. TB-Ag NPs have shown significantly in vitro anti-inflammatory activities by exhibiting dose-dependent NO and PGE 2 inhibitory activities with IC 50 values of 32.82 µg/mL and 67.25 µg/mL, respectively. This study concluded that the novel green synthesized TB-Ag NPs can be used as a potential novel anti-inflammatory and cytotoxicity agent to treat inflammatory-related diseases and inflammatory breast cancer (invasive ductal carcinoma) with biocompatible nature by targeting the tumor environment.