Characterization of citrus leaf blotch virus from Nandina domestica ‘Otafukunanten’

Nandina domestica ‘Otafukunanten’ shrubs with narrow-leaf symptoms were diagnosed with citrus leaf blotch virus (CLBV) infection based on RNA-sequencing. The full-length sequence of the CLBV strain isolated from N. domestica was determined, and the amino acid sequence shared 83.7% and 80.2% identity with the replicase polyprotein and coat protein, respectively, of the most closely related CLBV strain. All samples with narrow-leaf symptoms were infected with CLBV and cucumber mosaic virus. To the best of our knowledge, this is the first report of CLBV infection of a plant in the order Ranunculales.