Trpm5 channels encode bistability of spinal motoneurons and ensure motor control of hindlimbs in mice
NATURE COMMUNICATIONS(2021)
摘要
Bistable motoneurons of the spinal cord exhibit warmth-activated plateau potential driven by Na + and triggered by a brief excitation. The thermoregulating molecular mechanisms of bistability and their role in motor functions remain unknown. Here, we identify thermosensitive Na + -permeable Trpm5 channels as the main molecular players for bistability in mouse motoneurons. Pharmacological, genetic or computational inhibition of Trpm5 occlude bistable-related properties (slow afterdepolarization, windup, plateau potentials) and reduce spinal locomotor outputs while central pattern generators for locomotion operate normally. At cellular level, Trpm5 is activated by a ryanodine-mediated Ca 2+ release and turned off by Ca 2+ reuptake through the sarco/endoplasmic reticulum Ca 2+ -ATPase (SERCA) pump. Mice in which Trpm5 is genetically silenced in most lumbar motoneurons develop hindlimb paresis and show difficulties in executing high-demanding locomotor tasks. Overall, by encoding bistability in motoneurons, Trpm5 appears indispensable for producing a postural tone in hindlimbs and amplifying the locomotor output.
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关键词
Intrinsic excitability,Ion channels in the nervous system,Spinal cord,Science,Humanities and Social Sciences,multidisciplinary
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