Biomass sugar-powered enzymatic fuel cells based on a synthetic enzymatic pathway

Biomass stores a tremendous amount of chemical energy and is considered as an abundant and sustainable alternative to fossil fuels. However, the use of biomass to produce mW-level electricity for portable devices suffers from its structural complexity and therefore low energy conversion efficiency. In this study, we design an enzymatic pathway that could co-utilize and completely oxidize glucose and xylose from biomass hydrolysate to achieve high energy density in EFC. Faraday efficiency of 92% and maximum power density of 0.14 mW cm(-2) are achieved in this EFC. After the systematically optimization of enzyme loading and temperature as well as the removal of enzyme inhibitor from biomass hydrolysate by activated charcoal, the biomass sugar-powered EFC could reach a maximum power density of 0.5 mW cm(-2) and remain 60% of the initial value after 10 days. These results offer a feasible way to extract the energy stored in biomass as much as possible without the side effects of biomass hydrolysate on EFC. (C) 2021 Elsevier B.V. All rights reserved.