Analytical characterization of the SARS-CoV-2 EURM-017 reference material

•EURM-017 is human serum reference material for anti-SARS-CoV-2 serology assays.•Antibody fractions (S1 RBD, N, S1, S2, and full-length S) were affinity purified.•The five purified anti-sera were quantified, and neutralization activity compared.•Anti-sera ug/mL yield was: S1(17.7), S1 RBD(17.4), full-length S(34.1), S2(29.7), N(72.5).•Standardization is for assays with same antigen specificity/immunoglobulin class.•EURM-017 standardization will provide confidence to compare results across assays.