MINFLUX single particle tracking at exceptional spatial and temporal resolution

MINFLUX can localize single molecules by measuring the fluorescence signal with an excitation beam featuring an intensity zero placed to pre-defined positions in the fluorophore’s immediate proximity. Thus, the number of fluorescence photons needed to localize the molecule is minimized. MINFLUX affords fluorophore localization with a very small number of detected photons and consequently within a spatio-temporal regime exceeding that of alternative techniques [1].