Effect of Bone Marrow Derived Mesenchymal Stem Cells on Albino Rats, Submandibular Salivary Gland After Intraglandular Injection of Bleomycin

Objective: To evaluate effect of bone marrow mesenchymal stem cells (BMMSCS) on albino rats submandibular salivary gland after intraglandular injection of bleomycin (BLM). Methods: Fortytwo rats were randomly divided into three groups. Group I (n=18): was subjected to intraglandular injection of a single dose of 1.5mg/kg of bleomycin. Group II (n=18): was subjected to intraglandular injection of a single dose of 1.5mg/kg of bleomycin dissolved in distilled water. After four weeks; rats were subjected to another injection of (1 x 10 ) cells of BMMSCs. Group III (n=6): was subjected to intraglandular injection of 0.05 ml of distilled water in the left submandibular salivary gland and rats were sacrificed after five weeks from starting the study. Tissue sections were stained with Toluidine blue then were subjected to digital image analysis followed by oneway ANOVA statistical analysis. Effect of Bone Marrow Derived Mesenchymal Stem Cells on Albino Rats Submandibular Salivary Gland After Intraglandular Injection of Bleomycin Mansoura Journal of Dentistry 2019;6(24):1-3. 2 Esraa Abd Elraouf Elsayed Ahmed Rats were subjected to intraglandular injection of a single dose of 1.5mg/kg of bleomycin dissolved in distilled water in the left submandibular salivary gland; then after four weeks six rats were sacrificed at 3 different times at 3, 7, 14 days. Group II: (n=18) Rats of this group were subjected to intraglandular injection of a single dose of 1.5mg/kg (124) of bleomycin dissolved in distilled water. After four weeks; rats were subjected to another injection of (1 x 10 ) cells of BMMSCs . six rats were sacrificed at 3, 7, 14 days from BMMSCs injection. Group III: (n=6) Rats of this group were subjected to intraglandular injection of 0.05 ml of distilled water in the left submandibular salivary gland. (sham group) They were sacrificed after four weeks. Results: Group I: revealed ductal and acinar degeneration with loss of adhesion and loss of metachromatic secretory granules. Group II: revealed more or less restoring ordinary architecture with maintenance distribution and adhesion of acini and large amount of metachromatic secretory granules. Group III: ordinary architecture with maintenance distribution and adhesion of acini and large amount of metachromatic secretory granules. Photomicrograph of group I showing loss of metachromatic secretory granules Photomicrograph of group II showing very large amount of metachromatic secretory granules Mansoura Journal of Dentistry 2019;6(24):1-3. 3 Esraa Abd Elraouf Elsayed Ahmed Oneway ANOVA analysis revealed significant difference between groups. Table(1):Comparison of +ve cells between different studied groups. Group I Group II Group III ANOVA P value