Co-Modification With Mesenchymal Stem Cells Membrane and PDA Prevents Fe3O4-Induced Pulmonary Toxicity in Micevia AMPK-ULK1 Axis

Abstract Background: Fe 3 O 4 nanoparticles are widely used in the diagnosis and treatment of diseases, but the toxicity should not be ignored. It has been reported that PDA modification can reduce the toxicity of Fe 3 O 4 and increase the biocompatibility. However, a better modification method is still worth studying. We have developed a new method to coat Fe 3 O 4 @PDA nanoparticles with mesenchymal stem cells membrane (MSCM) and evaluated the lung toxicity of the modified particles to mice. Result: We found that MSCM modification significantly reduced the lung injury induced by Fe 3 O 4 nanoparticles in mice. Compared with Fe 3 O 4 @PDA nanoparticles, co-modification with MSCM and PDA modification significantly reduced autophagy and apoptosis of mouse lung tissue, and reduced the activation of autophagy pathway AMPK-ULK1 axis. Thus, co-modification with MSCM and PDA prevents Fe 3 O 4 -induced pulmonary toxicity in mice by inhibiting the AMPK-ULK1 derived autophagy. Conclusion: MSCM coated Fe 3 O 4 @PDA nanoparticles were demonstrated to prevent lung damage from autophagy and reduce the toxicity of iron oxide nanomaterials. The co-modification of PDA and MSCM can improve the biocompatibility and facilitate their further bioapplication.