Presence of SARS-CoV-2-reactive T cells in COVID-19 patients and healthy 1 donors 2 3

1 donors 2 3 Julian Braun*1,2, Lucie Loyal*1,2, Marco Frentsch*3, Daniel Wendisch4, Philipp Georg4, Florian 4 Kurth4, Stefan Hippenstiel4, Manuela Dingeldey1,2, Beate Kruse1,2, Florent Fauchere1,2, Emre 5 Baysal1,2, Maike Mangold1,2, Larissa Henze1,2, Roland Lauster1,5, Marcus A. Mall6,12, Kirsten 6 Beyer6, Jobst Röhmel6, Jürgen Schmitz7, Stefan Miltenyi7, Ilja Demuth8, Marcel A. Müller9, 7 Martin Witzenrath4, Norbert Suttorp4, Florian Kern10,11, Ulf Reimer10, Holger Wenschuh10, 8 Christian Drosten9,12, Victor M. Corman9, Claudia Giesecke-Thiel13§#, Leif Erik Sander4§# and 9 Andreas Thiel1,2§# 10 11 *these authors contributed equally to this work 12 §these authors co-supervised the study 13 #address correspondence to: 14 andreas.thiel@charite.de 15 leif-erik.sander@charite.de 16 giesecke@molgen.mpg.de 17 18 19 1Si-M / “Der Simulierte Mensch” a science framework of Technische Universität Berlin and 20 Charité Universitätsmedizin Berlin, Berlin, Germany 21 2Regenerative Immunology and Aging, BIH Center for Regenerative Therapies, Charité 22 Universitätsmedizin Berlin, Berlin, Germany 23 3Department of Hematology, Oncology and Tumor Immunology, Charité Universitätsmedizin 24 Berlin 25 4Department of Infectious Diseases and Respiratory Medicine, Charité Universitätsmedizin 26 Berlin 27 5Medical Biotechnology, Institute for Biotechnology, Technische Universität Berlin 28 6Department of Pediatric Pulmonology, Immunology and Critical Care Medicine, Charité 29 Universitätsmedizin Berlin 30 7Miltenyi Biotec GmbH, Bergisch-Gladbach, Germany 31 8Interdisciplinary Metabolism Center, Biology of Aging (BoA)-group, Charité 32 Universitätsmedizin Berlin 33 9Institute of Virology, Charité Universitätsmedizin Berlin, Berlin, Germany 34 10JPT Peptide Technologies GmbH, Berlin, Germany 35 11Brighton and Sussex Medical School, Department of Clinical and Experimental Medicine, 36 Brighton, UK 37 12Berlin Institute of Health (BIH), Berlin, Germany 38 13Max Planck Institute for Molecular Genetics, Berlin, Germany 39 40 41 . CC-BY-NC-ND 4.0 International license It is made available under a is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. (which was not certified by peer review) The copyright holder for this preprint this version posted April 22, 2020. ; https://doi.org/10.1101/2020.04.17.20061440 doi: medRxiv preprint