Isolation of nuclei from frozen human skeletal muscle for single nucleus RNA and chromatin assays v1

Dinh H H. Diep,James R.s. Hocker,Sebastian Preissl

semanticscholar（2021）

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摘要

This protocol was adapted for the isolation of single nuclei from frozen skeletal muscle tissues for molecular characterization with the SNARE-seq2 assay. References: (1) Preissl et al (2015). Circulation Research. Doi: 10.1161/CIRCRESAHA.115.306337 (2) Hocker et al (2021). Science Advances. Doi: 10.1126/sciadv.abf1444

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