Reprogramming fibroblasts and mononuclear blood cells into iPSCs using Sendai virus v1

Reprogramming primary blood mononuclear suspension cells into induced pluripotent stem cells using a non-integrating RNA viral (Sendai) reprogramming factor delivery method. This ™ CytoTune-iPS 2.0 is a self-replicating RNA virus-based reprogramming approach which utilizes 3 pre-packaged viral particles expressing human KLF-4+OCT4/POU5F1+SOX2, hKLF-4 and hc-Myc proteins respectively. Use of Sendai viral particles for delivery allows for an efficient non-viral reprogramming of suspension blood and a wide range of other cells into induced pluripotent stem cells (iPSCs). This protocol describes the preparation of primary cell (blood-derived) cultures and their subsequent reprogramming into iPSCs using Sendai virus transduction.