Gut Microbiota: characterization in some patients with active digestive disorders

Gut bacterial characterization could be a good criterion for prevention, diagnosis confirmation and monitoring of digestive diseases. Aim. To begin the first step for future establishment of the metagenomic methodology in Balearic healthy system for the characterization gut microbiota, in colorectal exploration of patients affected by bowel diseases. Material and methods. The bacteria were characterized in colorectal biopsy and brushing (healthy and inflamed regions) from the bowel by 16S rRNA gene V3-V4 regions and Nextera XT metagenomic techniques (Illumina). DNA was isolated by colonoscopy exploration from 10 subjects (20-75 years, both sex) who felt some symptoms of abdominal pain, diarrhea, episodic rectal bleeding. Gene libraries were prepared and sequenced for 451 bacterial genera. We evaluated the qualitative of food consumption as a complement. The results were analyzed by SPSS v.21. Results. Our results showed that the quality control of libraries and sequencing was adequate. There was great bacterial variability among subjects and sampling areas. PCA obtained showed that, the microbiota of patients was clustered together by disease type and the bacteria diversity of No evidence of active pathology patients clustered closely to ulcerative colitis distribution. Our preliminaries results suggested that the colorectal exploration is not enough for a complete diagnosis and follow-up of treatments. Conclusions. The first step to establish gut microbiota metagenomic technology in our group allowed us to obtain bacteria genomic libraries by colonoscopy exploration; from patient with bowel diseases; according to Illumina requirements. Preliminary results will permit to introduce the methodology; increasing the subject number and samples and nutritional characterization.