Recovery of conformational continuum from single-particle cryo-EM data: Optimization of ManifoldEM informed by ground-truth studies

Recovery of conformational continuum from single-particle cryo-EM data: Optimization of ManifoldEM informed by ground-truth studies Evan Seitz,1 Peter Schwander,2 Francisco Acosta-Reyes,3 Suvrajit Maji,3 and Joachim Frank1, 3 1) Columbia University, Department of Biological Sciences, New York, NY 10032, USA 2) University of Wisconsin-Milwaukee, Department of Physics, 3135 N. Maryland Ave, Milwaukee, WI 53211, USA 3) Columbia University Medical Center, Department of Biochemistry and Molecular Biophysics, New York, NY 10032