Simultaneous RNA and protein profiling using a TurboID proximity-labeling strategy.

Our novel TurboID proximity labeling approach can simultaneously capture cell type-specific transcriptomes and proteomes. We are now validating this method in other cell types using RNA-sequencing and MS approaches. Once validated, this concurrent RNA and protein profiling approach can be applied to in vivo and ex vivo model systems to investigate the distinct roles brain cell types play in development, aging, and disease.